Characterisation of anthocyanins derived from carrot (Daucus carota) cell culture

Two anthocyanin pigments were isolated from cell cultures of Carrot, Nentes scarlet-104 local variety. Chemical hydrolysis, column and paper chromatography, HPLC, proton and C-13 NMR and mass spectroscopic studies indicated the presence of cyanidin-3-lathyroside [cyanidin-3-O{beta-D-xylopyranosyl (1-->2) beta-D-galacto pyranoside}] (90%) and cyanidin-3-beta-D-glucopyranoside (10%) in the callus cultures, whereas only cyanidin-3-lathyroside (0.05%) was found in the explant carrot. The significant difference was that there was no acylated anthocyanin present as reported in other varieties of carrot. (C) 2000 Elsevier Science Ltd. All rights reserved.