期刊
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
卷 12, 期 1-2, 页码 29-32出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0928-4931(00)00153-3
关键词
SNOM; AFM; fluorescence; optical fiber; probe; DNA; YOYO-1
We utilized a novel scanning near-field optical microscope (SNOM) for imaging DNA molecules. The microscope system was constructed with a commercial inverse microscope and a newly developed scanning unit. In the system, a bent optical fiber probe is used to operate in a dynamic mode atomic-force microscope (AFM). A lambda DNA solution of 5 mu M (base) with 5 mu M and 500 nM YOYO-1 was prepared and cast on a gamma-APTES treated cover slip. The lambda DNA was aggregated in line and immobilized on the cover slip. The percentage of fluorescence intensity of lambda DNA with 5 mu M YOYO-1 showed integers at almost each point on the DNA. As the fluorescence intensity correlated with the areas of a cross-section of the DNA topography, it appeared that YOYO-1 intercalated in the DNA homogeneously. The fluorescence images of lambda DNA with 500 mu M YOYO-1, however, were irregular and did not correlate with the area of the topographic cross-section, suggesting that YOYO-1 did not intercalate in the lambda DNA uniformly in this concentration and intercalated cooperatively. (C) 2000 Elsevier Science S.A. All rights reserved.
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