期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 275, 期 33, 页码 25465-25470出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M000903200
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资金
- NIAMS NIH HHS [AR17803] Funding Source: Medline
- NICHD NIH HHS [U54 HD12629] Funding Source: Medline
Pituitary gonadotropes transduce hormonal input into cytoplasmic calcium ([Ca2+](cyt)) oscillations that drive rhythmic exocytosis of gonadotropins, Using Calcium Green-1 and rhod-2 as optical measures of cytoplasmic and mitochondrial free Ca2+, we show that mitochondria sequester Ca2+ and tune the frequency of [Ca2+](cyt) oscillations in rat gonadotropes. Mitochondria accumulated Ca2+ rapidly and in phase with elevations of [Ca2+](cyt) after GnRH stimulation or membrane depolarization. Inhibiting mitochondrial Ca2+ uptake by the protonophore CCCP reduced the frequency of GnRH-induced [Ca2+](cyt) oscillations or, occasionally, stopped them. Much of the Ca2+ that entered mitochondria is bound by intramitochondrial Ca2+ buffering systems. The mitochondrial Ca2+ binding ratio may be dynamic because [Ca2+](mit), appeared to reach a plateau as mitochondrial Ca2+ accumulation continued. Entry of Ca2+ into mitochondria was associated with a small drop in the mitochondrial membrane potential. Ca2+ was extruded from mitochondria more slowly than it entered, and much of this efflux could be blocked by CGP-37157, a selective inhibitor of mitochondrial Na+-Ca2+ exchange. Plasma membrane capacitance changes in response to depolarizing voltage trains were increased when CCCP was added, showing that mitochondria lower the local [Ca2+](cyt) near sites that trigger exocytosis. Thus, we demonstrate a central role for mitochondria in a significant physiological response.
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