期刊
FEBS LETTERS
卷 479, 期 3, 页码 127-130出版社
WILEY
DOI: 10.1016/S0014-5793(00)01895-0
关键词
green fluorescent protein; red fluorescent protein; random mutagenesis; drFP593; drFP583
A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm, Using the remarkable similarity of the drFP583 and dsFP593 genes, we performed a 'shuffling' procedure to generate a pool of mutants consisting of various combinations of parts of both genes. One 'hybrid gene' was chosen for subsequent random mutagenesis, which resulted in a mutant variant with a uniquely red-shifted emission maximum at 616 nm, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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