期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 242, 期 1-2, 页码 193-204出版社
ELSEVIER
DOI: 10.1016/S0022-1759(00)00201-5
关键词
E. coli; heat-induction; soluble; antibody; multimers; scFv
Multivalent antibody fragments (scFv dimers, trimers and tetramers) provide high avidity and ideal pharmacokinetics for tumour targeting applications. This protocol describes our optimised protocol for high-level bacterial synthesis of soluble antibody scFv fragments, as either monomers or multimers, using the heat-inducible bacterial expression vector pPOW3. Our protocol is rapid, which minimizes protein degradation, and utilises inexpensive reagents for cost-effective product synthesis. The strong, temperature-regulated promoters in pPOW3 provide efficient production of either monomeric or multimeric single-chain antibody fragments as dictated by the gene construct design. (C) 2000 Elsevier Science B.V. All rights reserved.
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