期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 97, 期 18, 页码 10101-10106出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.97.18.10101
关键词
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资金
- NCI NIH HHS [R01 CA077097] Funding Source: Medline
- NHGRI NIH HHS [K01 HG000038, 1 K01 HG00038-01] Funding Source: Medline
We describe the use of singular value decomposition in transforming genome-wide expression data from genes x arrays space to reduced diagonalized eigengenes x eigenarrays space, where the eigengenes (or eigenarrays) are unique orthonormal superpositions of the genes (or arrays). Normalizing the data by filtering out the eigengenes land eigenarrays) that are inferred to represent noise or experimental artifacts enables meaningful comparison of the expression of different genes across different arrays in different experiments. Sorting the data according to the eigengenes and eigenarrays gives a global picture of the dynamics of gene expression, in which individual genes and arrays appear to be classified into groups of similar regulation and function, or similar cellular state and biological phenotype, respectively. After normalization and sorting, the significant eigengenes and eigenarrays can be associated with observed genome-wide effects of regulators, or with measured samples, in which these regulators are overactive or underactive, respectively.
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