期刊
JOURNAL OF APPLIED ANIMAL RESEARCH
卷 18, 期 1, 页码 73-80出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09712119.2000.9706325
关键词
Buffalo; rumen contents; carboxymethylcellulase; alpha-amylase; xylanase; microcrystalline cellulase; filter paper degrading activity; rumen enzymes
The hydrolytic enzymes like carboxymethylcellulase, alpha-amylase, xylanase, microcrystalline cellulase and filter paper degrading activities were estimated in various fractions of rumen contents of Murrah buffalo. About 80-92% of the enzyme activities were associated with the particulate material, while only a negligible portion. (1-4%) was present as extracellular fraction in the liquid portion of rumen contents. Rest of the activities (8-15%) were associated with freely suspended microbial cells irt the liquid portion of the rumen contents. Maximum activities of carboxymethylcellulase, alpha-amylase, xylanase and micro-crystalline cellulase were observed at 2h post feeding while in the case of filter paper degrading activity, there was no difference in the activities in the first 4 hours of offering feed to the animals.
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