期刊
JOURNAL OF VIROLOGY
卷 74, 期 18, 页码 8472-8479出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.18.8472-8479.2000
关键词
-
类别
资金
- NIAID NIH HHS [R01 AI038296, R37 AI038296, AI38296] Funding Source: Medline
The reovirus attachment protein, sigma 1, is responsible for strain-specific patterns of viral tropism in the murine central nervous system and receptor binding on cultured cells, The sigma 1 protein consists of a fibrous tail domain proximal to the virion surface and a virion-distal globular head domain. To better understand mechanisms of reovirus attachment to cells, we conducted studies to identify the region of sigma 1 that binds cell surface carbohydrate. Chimeric and truncated sigma 1 proteins derived from prototype reovirus strains type 1 Lang (TLL) and type 3 Dearing (T3D) were expressed in insect cells by using a baculovirus vector. Assessment of expressed protein susceptibility to proteolytic cleavage, binding to anti-sigma 1 antibodies, and oligomerization indicates that the chimeric and truncated ol proteins are properly folded. To assess carbohydrate binding, recombinant sigma 1 proteins were tested for the capacity to agglutinate mammalian erythrocytes and to bind sialic acid presented on glycophorin, the cell surface molecule bound by type 3 reovirus on human erythrocytes, Using a panel of two wild-type and ten chimeric and truncated ol proteins, the sialic acid-binding domain of type 3 sigma 1 was mapped to a region of sequence proposed to form the more amino terminal of two predicted beta-sheet structures in the tail. This unit corresponds to morphologic region T(iii) observed in computer-processed electron micrographs of sigma 1 protein purified from virions, In contrast, the homologous region of T1L sigma 1 sequence was not implicated in carbohydrate binding; rather, sequences in the distal portion of the tail known as the neck were required. Results of these studies demonstrate that a functional receptor-binding domain, which uses sialic acid as its ligand, is contained within morphologic region T(iii) of the type 3 sigma 1 tail. Furthermore, our findings indicate that T1L and T3D sigma 1 proteins contain different arrangements of receptor-binding domains.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据