期刊
ANALYTICA CHIMICA ACTA
卷 885, 期 -, 页码 98-105出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.05.025
关键词
Polypyrrole-coated nanoparticles; Magnetic solid phase extraction; Filtration-based phase separation; High performance liquid chromatography; Megestrol acetate; Levonorgestrel
资金
- Tarbiat Modares University
The aim of the present work is combination of the advantages of magnetic solid phase extraction (MSPE) and dispersive liquid phase microextraction (DLLME) followed by filtration-based phase separation. A new pretreatment method was developed for trace determination of megestrol acetate and levonorgestrel by liquid chromatography/ultraviolet detection in biological and wastewater samples. After magnetic solid phase extraction, the eluent of MSPE was used as the disperser solvent for DLLME. Emulsion resulted from DLLME procedure was passed through the in-line filter for phase separation. Finally the retained analytes in the filter was washed with mobile phase of liquid chromatography and transferred to the column for separation. This approach offers the preconcentration factors of 3680 and 3750 for megestrol acetate and levonorgestrel, respectively. This guarantees determination of the organic compounds at trace levels. The important parameters influencing the extraction efficiency were studied and optimized. Under the optimal extraction conditions, a linear range of 0.05-50 ng mL(-1) (R-2 > 0.998) and limit of detection of 0.03 ng mL(-1) were obtained for megestrol acetate and levonorgestrel. Under optimal conditions, the method was successfully applied for determination of target analytes in urine and wastewater samples and satisfactory results were obtained ( RSDs < 6.8%). (C) 2015 Elsevier B.V. All rights reserved.
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