期刊
BIOLOGICAL CONTROL
卷 57, 期 3, 页码 208-214出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.biocontrol.2011.02.012
关键词
Pichia guilliermondii; Penicillium digitatum; Biocontrol; EST; Green mold
资金
- Instituto Politecnico Nacional (PIFI-IPN) [SIP2007-0786]
To gain insight into the antagonistic molecular mechanisms displayed by the biocontrol epiphytic-yeast Pichia guilliermodii (isolate LCBG-03), its patterns of gene expression were evaluated during direct and indirect induction with Penicillium digitatum EfiA, a well-known fungal phytopathogen during the postharvest processing of citrus. Assays of in vitro antagonistic activity indicated a strong inhibitory effect on fungal growth and spore germination by the yeast, even when yeast-fungus interaction was performed on a rich medium. Antagonist gene expression was evaluated in induced condition as well as in direct interaction using differential expressed sequence tags (ESTs) obtained by two methodologies; suppression subtractive hybridization (SSH) and differential display (DD). We obtained the genetic response of the yeast under three different specific metabolic conditions: starvation by carbon source competence, sensing of extracellular metabolites produced by active mycelium of P. digitatum (membrane system) and induction by fungal cell walls. As a result, we observed just one EST, associated as expected to energy metabolism in starvation conditions; for the membrane system, seven ESTs were obtained by the SSH methodology, all related with some of the following metabolic networks: energy, nitrogen, cell cycle, ABC transporters, response to stress and one unknown sequence. The induced (fungal cell walls) system produced the highest number of ESTs, with a total of 22, including all the metabolic networks mentioned above for the membrane system plus ESTs associated with signal transduction. (c) 2011 Elsevier Inc. All rights reserved.
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