期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 17, 期 9, 页码 1371-1383出版社
OXFORD UNIV PRESS
DOI: 10.1093/oxfordjournals.molbev.a026420
关键词
cytosine deamination; 5-methylcytosine deamination; mammalian genes; homeothermy; isochore evolution; computational molecular biology
DNA melting is rate-limiting for cytosine deamination, from which we infer that the rate of cytosine deamination should decline twofold for each 10% increase in GC content. Analysis of human DNA sequence data confirms that this is the case for 5-methylcytosine. Several lines of evidence further confirm that it is also the case for unmethylated cytosine and that cytosine deamination causes the majority of all C-->T and G-->A transitions in mammals. Thus, cytosine deamination and DNA base composition each affect the other, forming a positive feedback loop that facilitates divergent genetic drift to high or low CC content. Because a 10 degrees C increase in temperature in vitro increases the rate of cytosine deamination 5.7-fold, cytosine deamination must he highly dependent on body temperature, which is consistent with the dramatic differences between the isochores of warm-blooded versus cold-blooded vertebrates. Because this process involves both DNA melting and positive feedback, it would be expected to spread progressively (in evolutionary time) down the length of the chromosome, which is consistent with the large size of isochores in modem mammals.
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