期刊
INFECTION AND IMMUNITY
卷 68, 期 9, 页码 5450-5453出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.68.9.5450-5453.2000
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资金
- NHLBI NIH HHS [HL-55660] Funding Source: Medline
- NIAID NIH HHS [AI-16242, AI-39067, R29 AI040262] Funding Source: Medline
Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jejuni strain 480. Insertions were random, as demonstrated by Southern blot analysis and insertional junction sequencing. We have demonstrated, for the first time, random in vivo transposon mutagenesis of C. jejuni.
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