期刊
SCIENCE
卷 289, 期 5485, 页码 1757-1760出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.289.5485.1757
关键词
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资金
- NIGMS NIH HHS [GM 57356] Funding Source: Medline
A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the target; from an array substrate. This difference permits the discrimination of an oligonucleotide sequence from targets with single nucleotide mismatches with a selectivity that is over three times that observed for fluorophore-labeled targets. In addition, when coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude.
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