期刊
EMBO JOURNAL
卷 19, 期 18, 页码 4895-4902出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/19.18.4895
关键词
mitochondria; protein folding; protein translocation; TOM complex
A multisubunit complex in the mitochondrial outer membrane, the TOM complex, mediates targeting and membrane translocation of nuclear-encoded preproteins. We have isolated the TOM hole complex, containing the preprotein receptor components Tom70 and Tom20, and the TOM core complex, which lacks these receptors, The interaction of recombinant mitochondrial preproteins with both types of soluble TOM complex was analyzed. Preproteins bound efficiently in a specific manner to the isolated complexes in the absence of chaperones and lipids in a bilayer structure. Using fluorescence correlation spectroscopy, a dissociation constant in the nanomolar range was determined. The affinity was lower when the preprotein was stabilized in its folded conformation. Following the initial binding, the presequence was transferred into the translocation pore in a step that required unfolding of the mature part of the preprotein. This translocation step was also mediated by protease-treated TOM hole complex, which contains almost exclusively Tom40, Thus, the TOM core complex, consisting of Tom40, Tom22, Tom6 and Tom7, is a molecular machine that can recognize and partially translocate mitochondrial precursor proteins.
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