期刊
JOURNAL OF CELL BIOLOGY
卷 150, 期 6, 页码 1311-1320出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.150.6.1311
关键词
prenylation; membrane; GBD; neutrophil; thymus
类别
资金
- NCI NIH HHS [CA14195] Funding Source: Medline
- NCRR NIH HHS [RR00833] Funding Source: Medline
- NIGMS NIH HHS [GM26338] Funding Source: Medline
We purified native WASp (Wiskott-Aldrich Syndrome protein) from bovine thymus and studied its ability to stimulate actin nucleation by Arp2/3 complex. WASp alone is inactive in the presence or absence of 0.5 mu M GTP-Cdc42. Phosphatidylinositol 4,5 bisphosphate (PIP2) micelles allowed WASp to activate actin nucleation by Arp3/3 complex, and this was further enhanced twofold by GTP-Cdc42, Filaments nucleated by Arp2/3 complex and WASp in the presence of PIP2 and Cdc42 concentrated around lipid micelles and vesicles, providing that Cdc42 was GTP-bound and prenylated. Thus, the high concentration of WASp in neutrophils (9 mu M) is dependent on interactions with both acidic lipids and GTP-Cdc42 to activate actin nucleation by Arp2/3 complex. The results also suggest that membrane binding increases the local concentrations of Cdc42 and WASp, favoring their interaction.
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