期刊
ANALYTICA CHIMICA ACTA
卷 421, 期 2, 页码 147-153出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(00)01039-4
关键词
speciation; garlic; ES MS/MS; HPLC; ICP MS
Speciation of selenium in garlic harvested in naturally seleniferous soil was investigated. The sample was leached with water and the aqueous extract was fractionated by preparative size-exclusion chromatography. Selenium was found in only one (low-molecular mass) fraction. The chromatographic purity of the fraction was verified by reversed-phase chromatography with inductively coupled mass spectrometric detection. Again, one major signal accounting for more than 95% of the total selenium was observed. The heartcut fraction containing this compound produced an intense peak in an electrospray mass spectrum with the selenium pattern centered at m/z 313 (Se-80). Protonated molecular ions corresponding to the four Se isotopes gave rich fragmentation patterns by collision induced dissociation that allowed the identification of the selenium species to be gamma-glutamyl-Se-methylselenocysteine without the need for an authentic standard. (C) 2000 Elsevier Science B.V. All rights reserved.
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