期刊
AIDS
卷 14, 期 14, 页码 2109-2116出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00002030-200009290-00006
关键词
human herpesvirus 8; Kaposi's sarcoma; viral load; Africa
资金
- NCI NIH HHS [CA79389] Funding Source: Medline
Objective: To determine the relationship between human herpesvirus 8 (HHV-8 or Kaposi's sarcoma-associated herpesvirus) peripheral blood virus load and Kaposi's sarcoma (KS) clinical stage. Design: Blinded, cross-sectional analysis of peripheral blood HHV-8 DNA levels in persons with AIDS-related KS in Harare, Zimbabwe. Methods: Subjects were stratified by KS clinical stage. The amount of HHV-8 DNA in plasma and peripheral blood mononuclear cells (PBMC) was determined by quantitative real-time PCR amplification of the HHV-8 open reading frame 26. Results: Thirty-one HIV-1/HHV-8-coinfected persons were studied: 26 subjects had histologically confirmed KS tone stage II, 11 stage III and 14 stage IV) and five subjects had antibodies to HHV-8; but did not have KS. The age, CD4 lymphocyte count and plasma HIV-1 RNA levels were similar in all groups. HHV-8 DNA was detected in the plasma of all HHV-8-infected subjects (range < 2.4 to 5.2 log(10) copies/ml), but plasma HHV-8 DNA levels were not associated with KS disease stage. In contrast, the amount of HHV-8 DNA in PBMC (range < 0.7 to 4.5 log(10) copies/mug) was strongly associated with KS clinical stage (P = 0.005). Among stage IV KS cases, there was a linear relationship between plasma and PBMC HHV-8 DNA levels (r(2) = 0.42; P = 0.01). Conclusion: The strong association observed between the extent of KS disease and the levels of HHV-8 DNA in PBMC provides further evidence for a relationship between HHV-8 virus load and KS pathogenesis. (C) 2000 Lippincott Williams & Wilkins.
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