期刊
ANALYTICA CHIMICA ACTA
卷 891, 期 -, 页码 32-42出版社
ELSEVIER
DOI: 10.1016/j.aca.2015.06.049
关键词
Epigenetic code; Histone post-translational modifications (HPTMs); Mass spectrometry (MS); Readers of histone modifications
资金
- National Basic Research Program of China [2012cb910601, 2013cb910903]
- National Natural Science Foundation of China [21275077]
- Tianjin Municipal Science and Technology Commission [14JCYBJC24000]
The so-called readers of histone post-translational modifications (HPTMs) refer to proteins or complexes that are recruited to HPTMs thus eventually regulate gene transcription. To identify these readers, mass spectrometry plays an essential role following various enriching strategies. These enriching methods include the use of modified histone peptides/proteins or chemically synthesized histones/nucleosomes containing desired HPTMs to enrich the readers of HPTMs. Despite the peptide- or protein-based assay is straightforward and easy to perform for most labs, this strategy has limited applications for those weak or combinational interactions among various HPTMs and false-positive results are a potential big problem. While the results derived from synthesized histone proteins/nucleosomes is more reliable as it mimics the real chromatic conditions thus is able to analyze the binders of those cross-talked HPTMs, usually the synthesis is so difficult that their applications are impeded for high throughput analysis. In this review, an overview of these analytical techniques is provided and their advantages and disadvantages are discussed. (C) 2015 Elsevier B.V. All rights reserved.
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