期刊
JOURNAL OF VIROLOGY
卷 74, 期 19, 页码 9078-9082出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.19.9078-9082.2000
关键词
-
类别
While analyzing human cytomegalovirus (HCMV) gene expression in infected cells by RNA-specific nucleic acid sequence-based amplification (NASBA), positive results were observed for HCMV RNA encoded by several viral genes immediately after the addition of the virus. W-inactivated virus also gave a positive NASBA result without establishing active infection, suggesting that RNA was associated with the inoculum, Highly purified virions devoid of cellular contamination proved to be positive for viral RNA encoding both immediate-early (UL123) and late (UL65) gene products. Virion-associated RNA might be incorporated specifically or without selection during the virion assembly. in the latter case, cellular RNA would also be present in the virion. A high-abundant cellular RNA encoded by GAPDH and even UW RNA,, which is expressed at low levels, were detected in the virion fraction, whereas cellular DNA was absent. Virion fractionation revealed that cellular RNA was absent in purified de-enveloped capsids. In conclusion, cellular and viral RNA. was present between the capsid and envelope of the virion, whereas in the capsici only viral RNA could be detected. The results suggest that virion-associated viral and cellular RNA is incorporated nonspecifically during virion assembly.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据