期刊
INFECTION AND IMMUNITY
卷 68, 期 10, 页码 5587-5594出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.68.10.5587-5594.2000
关键词
-
资金
- NIAID NIH HHS [R01 AI026328, R21 AI026328, AI-26328] Funding Source: Medline
The CD4 T helper cell type 1 (Th1) response is essential for the resolution of chlamydial genital infection in mice, However, not all Th1 clones are equally protective in eradicating the infection, Since oral immunization regimens produce protective immunity, we evaluated the role of the mucosa-associated homing receptor, alpha 4 beta 7, in trafficking to the genital mucosa, Using a panel of CD4, Th1 cell lines and clones, we compared the Lymphocyte homing patterns of a Chlamydia-specific, protective clone (P-MoPn), a nonprotective clone (N-MoPn), and a keyhole limpet hemocyanin (KLH)-specific cell line (KLH-1), T cells were labeled with the fluorescent dye PKH-26, adoptively transferred into Chlamydia-infected mice, and monitored at different time points throughout the course of a genital infection, We found that clones P-MoPn and N-MoPn migrated to similar extents to the genital tract and in significantly greater numbers than the KLH-specific T-cell line. Both clones and the KLH-1 line expressed similar levels of the adhesion molecules alpha 4, beta 1, CD44, and CD11a, However, clones P-MoPn and N-MoPn expressed higher levels of the mucosal homing receptor, alpha 4 beta 7, Also, clones P-MoPn and N-MoPn but not the KLH-1 line migrated to the mesenteric lymph node, suggesting a mucosal recirculation pattern, Moreover, blocking alpha 4 beta 7 adhesion interaction in vivo significantly reduced the recruitment of P-MoPn but not KLH-1 to the genital tract, These findings show that the mucosal homing receptor or4P7 is utilized by a subset of CD4 cells during migration to the Chlamydia-infected genital tract.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据