期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 62, 期 10, 页码 1109-1110出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.62.1109
关键词
amelogenin; equine sex-determination; SRY
A quick method for sex determination of horses was developed. Simultaneous amplification of the equine sex-determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) accomplished the determination of the presence of both the Y chromosome and SRY gene. In agarose gel electrophoresis, a normal stallion showed 1 SRY band and 3 AMEL (AMELX, AMELY, and AMELX/AMELY heteroduplex) bands, and a normal mare showed a single AMELX band. In XY-mares, 3 AMEL bands were detected as in a normal stallion, but no SRY band. The present method enables a quick diagnosis for XY-mare prior to cytogenetic analysis.
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