期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 41, 期 9, 页码 1311-1321出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.b18-00223
关键词
co-culture system; exosome; indirect co-culture
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [JP23234567, JP23591016]
- Kanazawa Medical University Research Project through the Private University Research Branding Project of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [RP2017-09]
- MEXT
- Kanazawa Medical University [S2013-8, S2014-7]
In biological systems, extracellular vesicles including exosomes have recently been revealed to play a significant role in the communication between various cells, and the number of papers on this subject has dramatically increased. In current conventional exosome studies, the standard research method is to use liquid biopsies to analyze extracts of various disease exosomes. However, exosomes are only one of many key players in natural cellular interactions. Reproducing the phenomena occurring in vivo and investigating the interactions are required in order to examine their role fully. For exosome research, an alternative to the liquid biopsy method for observing natural interactions is the co-culturing technique. It does not require an exosome extraction procedure, and while the technique has been used in many studies thus far, its application to exosome research has been limited. However, the use of co-culturing technologies is necessary to examine the essential interactions of exosomes. An overview of exosome research methodologies and co-culturing systems is thus provided here.
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