期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 35, 期 10, 页码 1669-1675出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.b12-00037
关键词
macelignan; matrix metalloproteinase-1; type I procollagen; mitogen-activated protein kinase; UV irradiation; reactive oxygen species
资金
- Yonsei Biomolecule Research Initiative of the two-step Brain Korea 21 Project
Exposure to ultraviolet (UV) light causes premature skin aging that is associated with upregulated matrix metalloproteinases (MMPs) and decreased collagen synthesis. Macelignan, a natural lignan compound isolated from Myristica fragrans HOUTT. (nutmeg), has been reported to possess antioxidant and antiinflammatory activities. This study assessed the effects of macelignan on photoaging and investigated its mechanisms of action in UV-irradiated human skin fibroblasts (Hs68) by reverse transcription-polymerase chain reaction, Western blot analysis, 2',7'-dichlorofluorescein diacetate assay, and enzyme-linked immunosorbent assay. Our results show that macelignan attenuated UV-induced MMP-1 expression by suppressing phosphorylation of mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Macelignan also increased type I procollagen expression and secretion through transforming growth factor beta (TGF-beta)/Smad signaling. These findings indicate that macelignan regulates the expression of MMP-1 and type I procollagen in UV-irradiated human skin fibroblasts by modulating MAPK and TGF-beta/Smad signaling, suggesting its potential as an efficacious antiphotoaging agent.
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