期刊
JOURNAL OF VIROLOGY
卷 74, 期 19, 页码 9152-9166出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.19.9152-9166.2000
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资金
- NIAID NIH HHS [AI-23173, R01 AI023173] Funding Source: Medline
- NIGMS NIH HHS [T32 GM-08152, T32 GM008152] Funding Source: Medline
Infection of cells by many viruses affects the cell division cycle of the host cell to favor viral replication. We examined the ability of the paramyxovirus simian parainfluenza virus 5 (SV5) to affect cell cycle progression, and we found that SV5 slows the;ate of proliferation of HeLa T4 cells. The SV5-infected cells had a delayed transition from G(1) to S phase and prolonged progression through S phase, and some of the infected cells were arrested in G(2) or M phase. The levels of p53 and p21(CIP1) were not increased in SV5-infected cells compared to mock-infected cells, suggesting that the changes in the cell cycle occur through a p53-independent mechanism. However, the phosphorylation of the retinoblastoma protein (pRB) was delayed and prolonged in SV5-infected cells. The changes in the cell cycle were also observed in cells expressing the SV5 V protein but not in the cells expressing the SV,5 P protein or the V protein lacking its unique C terminus (V Delta C). The unique C terminus of the V protein of SV5 was shown previously to interact dth DDB1, which is the 127-kDa subunit of the multifunctional damage-specific DNA-binding protein (DDB) heterodimer, The coexpression of DDB1 with V can partially restore the changes in the cell cycle caused bl expression of the V protein.
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