期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 34, 期 8, 页码 1287-1290出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.34.1287
关键词
trichoderin A; ATP synthase; anti-mycobacterial activity; aminolipopeptide; dormant; tuberculosis
资金
- Hoansha Foundation
- Uehara Memorial Foundation
- Naito Foundation
- JSPS-South Africa (NRF)
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Grants-in-Aid for Scientific Research [22659022, 21406002, 21310143, 23102005] Funding Source: KAKEN
In the course of our search for anti-dormant mycobacterial substances from marine organisms, we previously isolated three new aminolipopeptides, named trichoderins A, M and B, from the culture of the marine sponge-derived fungus of Trichoderma sp. and determined their chemical structures. To identify the gene that could confer a resistance to trichoderin A, we prepared transformants of Mycobacterium (M.) smegmatis, which were transformed with the genomic DNA library of M. bovis BCG constructed in the multi-copy shuttle cosmid pYUB145. Then, the transformant of M. smegmatis, which over-expressed a part of genes that coded mycobacterial ATP synthase, was found to exhibit a resistance to trichoderin A. In addition, trichoderin A reduced ATP contents in M. bovis BCG. These findings elucidated that the anti-mycobacterial activity of trichoderins comes from the inhibition of ATP synthesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据