期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 32, 期 7, 页码 1295-1297出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.32.1295
关键词
(1 -> 3)-beta-D-glucan; laminaran; nonlinear absorptive transport; nonlinear secretory transport; rat intestine
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18590156]
- Japan Health Sciences Foundation
- Grants-in-Aid for Scientific Research [18590156] Funding Source: KAKEN
The mechanism of the nonlinear concentration dependence of intestinal absorption of (1 -> 3)-beta-D-glucan was studied using in situ rat intestinal perfusion, as well as the in vitro Ussing-type chamber method mounted with rat intestinal tissue. The intestinal absorption rate constant of a (1 -> 3)-beta-D-glucan, laminaran, evaluated by the loop method increased significantly with increasing concentration of laminaran up to 0.5 mu M in a nonlinear fashion and tended to decrease at higher concentrations. Mucosal-to-serosal directed permeation of the laminaran across rat ileal sheets evaluated by the in vitro Ussing-type chamber method also decreased in a dose-dependent fashion. Serosal-to-mucosal directed permeation decreased in a concentration-dependent manner. In addition, the serosal-to-mucosal flux was reduced in the presence of metabolic inhibitor, 2,4-di-nitrophenol. These results suggest that laminaran is secreted into the intestinal lumen predominantly by the efflux transporting system. We conclude that intestinal transport of (1 -> 3)-beta-D-glucan involves specialized transporter or something similar in both absorptive and secretory directions, and complex nonlinear intestinal absorption characteristics can be ascribed to the participation of multiple transport mechanism.
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