期刊
NUCLEIC ACIDS RESEARCH
卷 28, 期 19, 页码 3785-3792出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/28.19.3785
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资金
- NIGMS NIH HHS [R01 GM043237, GM43237] Funding Source: Medline
Sixteen long-range crosslinks are induced in Escherichia coli 16S rRNA by far-UV irradiation. Crosslinking patterns in two other organisms, Bacillus subtilis and Thermos aquaticus, were investigated to determine if the number and location of crosslinks in E.coli occur because of unusually photoreactive nucleotides at particular locations in the rRNA sequence, Thirteen long-range crosslinks in B. subtilis and 15 long-range crosslinks in T. aquaticus were detected by gel electrophoresis and 10 crosslinks in each organism were identified completely by reverse transcription analysis. Of the 10 identified crosslinks in B. subtilis, eight correspond exactly to E.coli crosslinks and two crosslinks are formed close to sites of crosslinks in E.coli, Of the 10 identified crosslinks in T. aquaticus, five correspond exactly to E.coli crosslinks, three are formed close to E.coli crosslinking sites, one crosslink corresponds to a UV laser irradiation-induced crosslink in E,coli and the last is not seen in E,coli, The overall similarity of crosslink positions in the three organisms suggests that the crosslinks arise from tertiary interactions that are highly conserved but with differences in detail in some regions.
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