期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 32, 期 1, 页码 86-90出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.32.86
关键词
2,4,2',4'-tetrahydroxy-3-(3-methyl-2-butenyl)-chalcone; tyrosinase; melanin; melanin biosynthesis
资金
- National High Technology Research
- Development Program of China [2007AA02Z301]
- New Drug Basic Research Program of the Shanghai Institute of Materia Medica, Chinese Academy of Sciences [07G603L056]
2,4,2',4'-Tetrahydroxy-3-(3-methyl,1-2-butenyl)-chalcone (TMBC), a naturally occurring compound from Morus nigra, modulated melanogenesis by inhibiting tyrosinase. TMBC inhibited the L-dopa oxidase activity of mushroom tyrosinase with an IC50 value of 0.95 +/- 0.04 mu m, which was more potent than kojic acid (IC50 = 24.88 +/- 1.13 mu M), a well-known tyrosinase inhibitor. The kinetic studies of tyrosinase inhibition revealed that TMBC acts as a competitive inhibitor of mushroom tyrosinase with L-dopa as the substrate. Furthermore, TMBC effectively inhibited both cellular tyrosinase activity and melanin biosynthesis in B16 melanoma cells without significant cytotoxicity. The inhibitor), effect of TMBC on melanogenesis was attributed to the direct inhibition of tyrosinase activity, rather than the suppression of tyrosinase gene expression. These results indicated that TMBC may be a new promising pigmentation-altering agent for cosmetic or therapeutic applications.
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