Biological effects of a sulfated-polysaceharide isolated from the marine red algae Champia feldmannii

Sulfated-polysaccharides are exploited as antithrombotic and anticoagulant agents and suggested to be immunostimulants. The sulfated-polysaccharide isolated from the red-marine-algae Champia feldmannii (Cf-PLS) was purified by ion exchange chromatography and tested in experimental protocols of coagulation, inflammation (in Wistar rats) and nociception (in Swiss mice). Cf-PLS was tested Lv. for its anti-inflammatory activity in the paw-edema induced by classical inflammatory stimuli and s.c. for its pro-inflammatory activity in the paw-edema and peritonitis models. The anticoagulant activity was evaluated by the test of partial thromboplastin activation time (aPTT) and the antinociceptive effect in the writhing-test. Cf-PLS was not anti-inflammatory, but rather induced maximal edematogenic activity at 0.9mg/kg (1.01 +/- 0.030X0.06 +/- 0.03ml) compared to controls (0.06 +/- 0.03 ml), increased vascular-permeability (38.44 +/- 12.63 X 11.29 +/- 3.91 mu/g) and stimulated nentrophil migration (3.348 +/- 295X307 +/- 99cells/mu l) 1h after injection. Cf-PLS was also antinociceptive (6.6 +/- 1.28X33 +/- 1.44 writhes) and extended human plasma coagulation time by 3 times. Our data suggest that this molecule may be an important immunostimulant.