期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 31, 期 6, 页码 1250-1253出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.31.1250
关键词
tacrolimus; hematocrit value; overestimation; microparticle enzyme immunoassay; therapeutic drug monitoring
资金
- Japan Society the the Promotion of Science. [16922080]
The quality of microparticle enzyme immunoassay (MEIA) for blood tacrolimus is guaranteed in samples with hematocrit (Ht) values of 25 to 45%. Because MEIA provides inaccurate blood tacrolimus concentrations in samples with Fit out of this range (i.e. <25% or >45%), correction of the calibration is required for therapeutic drug monitoring. The authors demonstrated previously that overestimated MEIA tacrolimus concentration could be corrected by modified, calibrated MEIA (cMEIA) using the original calibrator. Here, an equation was established to more easily derive a corrected tacrolimus concentration by calculation (MEIAcalc) using the Fit of each sample. The tacrolimus concentrations of 99,whole-blood samples with low Fit (<25%) were then tested by the 3 assay methods: MEIA, cMEIA, and MEIAcalc. MEIA gave a significantly higher blood concentration of tacrolimus (median 12.9 ng/ml, range 3.6-26.4 ng/ml) than did cMEIA (median 10.0 ng/ml, range 0.2-21.1 ng/ml, p<0.05). This overestimation was eliminated by using MEIAcalc. There was no difference in blood tacrolimus concentration between cMEIA and MEIAcalc (median 10.0 ng/ml, range 1.7-21.41 ng/ml). MEIAcalc can be used to correct the tacrolimus concentration in samples obtained from patients with unstable Fit values.
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