期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 32, 期 10, 页码 1767-1778出版社
ELSEVIER SCI LTD
DOI: 10.1006/jmcc.2000.1211
关键词
nuclear factor-kappa B; angiotensin II; neonatal rat ventricular cardiomyocytes in culture; cell signaling; 92 kDa gelatinase; matrix metalloproteinase
Rat neonatal ventricular cardiomyocytes (RNVM) possess G protein-coupled AT(1) receptors for angiotensin II (AngII) that activate multiple intracellular pathways. To elucidate potential signaling mechanisms involved, we focussed on the nuclear transcription factor-kappa B (NF-kappaB) in RNVM culture. Using specific antibody to NF-kappa Bp65, immunolocalization of NF-kappaB was cytoplasmic in unstimulated cardiomyocytes, whereas NF-kappaB was translocated into the RNVM nucleus in response to AngII. This translocation was inhibited in the presence of calphostin C, a specific inhibitor of protein kinase C (PKC). Western blot analysis showed an increase of NF-kappaB in AngII-stimulated cardiomyocyte nuclear extracts as compared to controls, Biomolecular interaction analysis (BIA analysis) of NF-kappaB activation showed that only AngII-nuclear extracts bound to NF-kappaB consensus sequence with a high degree of affinity. This DNA-binding capacity was completely lost in calphostin C-treated cells. At transcriptional level in RNVM, AngII mediates the upregulation of matrix gelatinase (MMP-9), which is totally inhibited by calphostin C treatment. In conclusion, cardiomyocyte nuclear NF-kappaB translocation in response to Ang II via PKC pathway activates cardiomyocyte-specific transcription of MMP-9 and may activate transcription From responsive genes which are involved in cardiac hypertrophy process and/or cardiac remodeling. (C) 2000 Academic Press.
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