期刊
BONE MARROW TRANSPLANTATION
卷 26, 期 8, 页码 823-829出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.bmt.1702635
关键词
CD34(+) blood stem cells; magnetic-activated cell separation; allogeneic transplantation
The feasibility of transplantation using highly purified G-CSF-mobilized peripheral blood CD34(+) cells from HLA-identical sibling donors without prophylactic posttransplant immunosuppression was prospectively studied in 10 adult first chronic phase chronic myeloid leukemia (CML) patients with special reference to graft engineering performance and follow-up studies of minimal residual disease and immune reconstitution, CD34(+) cells were enriched by clinical-scale magnetic-activated cell separation (MACS) using iron-dextran beads bound to monoclonal anti-CD34 antibody, Grafts contained a median of 9.7 (range 1.7-16.6)x10(6) CD34(+) cells per kilogram of recipient body weight with a purity between 94.5% and 98.3% (median 97.2%), The median number of transfused CD3(+) T lymphocytes was 1.0 (range 0.5-8,5)x10(-4)/kg, corresponding to a log(10) T lymphocyte depletion between 3.8 and 5.0 (median 4.6). All patients engrafted rapidly with a median duration to neutrophil counts >500/mul of 8 (range 8-19) days and to self-sustaining platelet counts >20 000 mul of 12 (range 9-25) days, Isolated skin acute graft-versus-host disease (GVHD) of stages I to II occurred in three patients. One patient developed secondary graft failure and was successfully salvaged by an unmanipulated blood stem cell graft from the same donor. All 10 patients are surviving in complete hematologic, cytogenetic and molecular remission (four patients after donor lymphocyte infusions) between 12 and 22 (median 16) months post transplant, In conclusion, transplantation of MACS-purified blood CD34(+) cells from HLA-identical sibling donors in adult CML patients appears safe, effectively prevents acute GVHD without prophylactic post-transplant immunosuppression, and is capable of inducing complete cytogenetic and molecular remissions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据