期刊
BIOLOGIA PLANTARUM
卷 56, 期 3, 页码 451-457出版社
SPRINGER
DOI: 10.1007/s10535-012-0119-x
关键词
GDP-L-galactose guanyltransferase; transgenic plants; vitamin C
资金
- China National High-Tech 863 Program [2011AA100605]
- China Transgenic Research Program [2011ZX08002-001]
- Shanghai Leading Academic Discipline Project [B209]
Six genes, which encode enzymes involved in ascorbic acid (AsA) biosynthesis, including guanosine diphosphate (GDP)-mannose pyrophosphorylase (GMP), GDP-mannose-3',5'-epimerase (GME), GDP-galactose guanylyltransferase (GGT), L-galactose-1-phosphate phosphatase (GPP), L-galactose dehydrogenase (GDH) and L-galactono-1,4-lactone dehydrogenase (GLDH) were transformed into Arabidopsis thaliana, to evaluate the contribution of each gene to AsA accumulation. Additionally, two combinations, GGT-GPP and GGT-GLDH, were co-transformed into Arabidopsis with a reliable double-gene transformation system. AsA content of GGT transgenic lines was 2.9-fold higher as compared to the control, and co-transformation led up to 4.1-fold AsA enhancement. These results provided further evidence that GGT is the key enzyme in plant AsA biosynthesis.
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