期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 4, 期 5, 页码 573-580出版社
ELSEVIER SCI LTD
DOI: 10.1016/S1367-5931(00)00135-6
关键词
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Insights into glycosidase mechanisms have come from X-ray crystallographic studies on complexes with substrate analogs and inhibitors, representing all the intermediate species along the reaction coordinate. Site-directed mutagenesis continues to play a significant role in understanding mechanisms, but is also proving important in generating glycosidases of modified mechanism or specificity.
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