4.3 Article

Expression of the ndhCKJ operon of barley and editing at the 13th base of the mRNA of the ndhC gene

期刊

BIOLOGIA PLANTARUM
卷 52, 期 2, 页码 347-350

出版社

ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s10535-008-0071-y

关键词

herbicide; monocot; Hordeum vulgare; oxidative stress; plastid DNA; transcripts

向作者/读者索取更多资源

We have determined a 1778 base sequence which includes the complete ndhCKJ operon of barley plastid DNA. This operon contains the ndhC, ndhK and ndhJ genes encoding the polypeptides NDH-C, NDH-K and NDH-J, respectively, of the thylakoid Ndh complex. Poly-and mono-cistronic transcripts were identified, with an increase in the latter under oxidative stress induced by herbicide Paraquat. Complete sequencing of transcript cDNAs and of the corresponding regions of five additional monocots revealed that the 13(th) C (cytosine) base of ndhC is edited to U (uracil) converting the CAC codon (encoding histidine, H) to UAC (encoding tyrosine, Y). Dicots having the appropriate TAC codon at the genome sequence do not require editing. The new editing site can not be predicted by comparison with the Marchantia sequence (that has a C at the 13(th) position) because, in contrast to Angiosperms, the amino-terminal sequence in lower plants is highly variable in NDH-C.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据