期刊
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
卷 20, 期 10, 页码 2220-2225出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/01.ATV.20.10.2220
关键词
shear stress; smooch muscle cell; collagen gel; prostaglandin
资金
- NHLBI NIH HHS [HL-35549] Funding Source: Medline
A 3D collagen gel model was developed to simulate interstitial fluid flow and to assess the importance of this flow on the biochemical production rates of vascular smooth muscle cells (SMCs). Rat aortic SMCs were suspended in type I collagen, and the gel was supported by nylon fibers that allowed a 9-cm length of the SMC-gel model to withstand 90 cm H2O differential pressure over a 6-hour period without significant compaction. Up to 1 dyne/cm(2) shear stress on the suspended SMCs could be induced by the pressure-driven interstitial flow. The suspended SMCs were globular, had a diameter of approximate to 10 mum, and were distributed uniformly throughout the gel. The collagen fibers formed a network that was connected randomly with the surface of SMCs and nylon fibers. The diameter of the collagen fibers was approximate to 100 nm, and the concentration of collagen was 2.5 mg/mL. Using these parameters, fiber matrix theory predicted a Darcy permeability coefficient (K-p) of 1.22 x 10(-8) cm(2), which was close to the measured value of K-p. The production rates of prostaglandin (PG) I-2 and PGE(2) were used as markers of biochemical responsiveness of SMCs to fluid shear stress. Both PSI and PGE, production rates under 1 dyne/cm(2) shear stress were significantly elevated relative to static (no-flow) cont;ols. The production rates, however, were approximate to 10 times lower than observed when the same cells were plated on collagen-treated glass slides (2D model) and exposed to the same level of shear stress by use of a rotating disk apparatus. The results indicate that interstitial flow can affect SMC biology and that SMCs an more quiescent in 3D cultures than in 2D cultures. The 3D collagen gel model should be useful for future studies of interstitial now effects on SMC function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据