期刊
BIOLOGIA
卷 64, 期 2, 页码 319-323出版社
SPRINGER
DOI: 10.2478/s11756-009-0040-8
关键词
abscisic acid; Artemisia annua L.; artemisinin; cell suspension culture; HPLC-ELSD
类别
资金
- Shanghai Science and Technology Committee [08391911800]
- China National High-Tech 863 Program [2007AA10Z189]
- Shanghai Leading Academic Discipline Project [B209]
- [2007CB108805]
Artemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L., is the most effective antimalarial drug. In an effort to increase the artemisinin production, abscisic acid (ABA) with different concentrations (1, 10 and 100 A mu M) was tested by treating A. annua plants. As a result, the artemisinin content in ABA-treated plants was significantly increased. Especially, artemisinin content in plants treated by 10 A mu M ABA was 65% higher than that in the control plants, up to an average of 1.84% dry weight. Gene expression analysis showed that in both the ABA-treated plants and cell suspension cultures, HMGR, FPS, CYP71AV1 and CPR, the important genes in the artemisinin biosynthetic pathway, were significantly induced. While only a slight increase of ADS expression was observed in ABA-treated plants, no expression of ADS was detected in cell suspension cultures. This study suggests that there is probably a crosstalk between the ABA signaling pathway and artemisinin biosynthetic pathway and that CYP71AV1, which was induced most significantly, may play a key regulatory role in the artemisinin biosynthetic pathway.
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