期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 97, 期 21, 页码 11455-11459出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.97.21.11455
关键词
Alzheimer's disease; beta-amyloid; vaccine; EFRH phage; autoantibodies
The epitope EFRH. corresponding to amino acids 3-6 within the human beta-amyloid peptide (A beta P). acts as a regulatory site controlling both the formation and disaggregation process of the beta-amyloid fibrils (A beta). Locking of this epitope by highly specific antibodies affects the dynamics of the entire A beta P molecule, preventing self-aggregation as well as enabling resolubilization of already formed aggregates. Production of such antibodies by repeated injections of toxic human A beta fibrils into transgenic mice suggests the feasibility of vaccination against Alzheimer's disease. Here, we report the development of an immunization procedure for the production of effective anti-aggregating beta-amyloid antibodies based on filamentous phages displaying the EFRH peptide as specific and nontoxic antigen. Effective autoimmune antibodies were obtained by EFRH phage administration in guinea pigs. which exhibit A beta P identical to the human A beta P region. Moreover. because of the high antigenicity of the phage. no adjuvant is required to obtain high affinity anti-aggregating IgG antibodies after a short immunization period of 3 weeks. Availability of such antibodies opens up possibilities for the development of an efficient and long-lasting vaccination for the prevention and treatment of Alzheimer's disease.
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