期刊
GENES & DEVELOPMENT
卷 14, 期 20, 页码 2623-2634出版社
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.831900
关键词
Spt; P-TEFb; CTD; elongation; RNA polymerase; polytene
资金
- NIGMS NIH HHS [GM32967, GMOD5582, R37 GM032967, R01 GM032967] Funding Source: Medline
The Spt4, Spt5, and Spt6 proteins are consented throughout eukaryotes and are believed to play critical and related roles in transcription. They have a positive role in transcription elongation in Saccharomyces cerevisiae and in the activation of transcription by the HIV Tat protein in human cells. In contrast, a complex of Spt4 and Spt5 is required in vitro for the inhibition of RNA polymerase II (Pol II) elongation by the drug DRB, suggesting also a negative role in vivo. To learn more about the function of the Spt4/Spt5 complex and Spt6 in vivo, we have identified Drosophila homologs of Spt5 and Spt6 and characterized their localization on Drosophila polytene chromosomes. We find that Spt5 and Spt6 localize extensively with the phosphorylated, actively elongating form of Pol II, to transcriptionally active sites during salivary gland development and upon heat shock. Furthermore, Spt5 and Spt6 do not colocalize widely with the unphosphorylated, nonelongating form of pol II. These results strongly suggest that Spt5 and Spt6 play closely related roles associated with active transcription in vivo.
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