Amperometric glucose sensor based on glucose oxidase immobilized in electrochemically generated poly(ethacridine)

Ethacridine was successfully polymerized at a Pt electrode by potentiostatic and cyclic voltammetric methods. Glucose oxidase, as doped anions, was simultaneously incorporated into the matrixes of thin poly(ethacridine) (PETD), which was developed to fabricate a amperometric glucose sensor. This enzyme electrode exhibited a fast amperometric response to glucose within 2 s. The Michaelis-Menten constant K-m' and the maximum current density J(max), calculated from the response of glucose at this sensor, were 15.7 mM and 209 mu A/cm(2), respectively. Due to its selective permeability, PETD drastically reduced the effects of electroactive interference such as L-ascorbic acid, uric acid, o-acetaminophenol and L-cysteine. Furthermore, good stability was observed for this glucose sensor. (C) 2000 Elsevier Science B.V. All rights reserved.