期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 244, 期 1-2, 页码 185-193出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(00)00250-7
关键词
Cre recombinase; LoxP; gene exchange
资金
- NCI NIH HHS [CA16858] Funding Source: Medline
- NIAID NIH HHS [AI39187, AI29470] Funding Source: Medline
A novel method for the site-specific introduction of genes into eukaryotic cells using the prokaryotic Cre-LoxP recombination system is presented. Cre recombinase catalyzes recombination between two LoxP sites or between two mutant LoxP 511 sites, However, recombination is not catalyzed between a LoxP and a Loxp 511 site. We now demonstrate that it is possible to catalyze accurate exchange between two DNA segments each flanked by a LoxP and a LoxP 511 site. In the example presented, expression of the Cre recombinase resulted in the replacement of a murine IgA constant region gene with a LoxP site at the 5' end and a LoxP 511 site at the 3' end by a human IgA constant region gene flanked by the same wild type and mutant LoxP sites. This method provides a novel approach for the site-specific substitution of specific genes. (C) 2000 Elsevier Science B.V. All rights reserved.
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