期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 97, 期 22, 页码 12132-12137出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.97.22.12132
关键词
-
资金
- NIDDK NIH HHS [DK34987, P30 DK034987, R01-DK52851] Funding Source: Medline
An in vitro colony-forming assay and flow cytometry were used to identify rat hepatoblasts as being classical MHC class I, RT1A(I-)OX18(low) intercellular adhesion molecule 1 (ICAM-1)(+). Inducible differentiation toward biliary lineage was observed in most colonies derived from single RT1A(I-) progenitors, proving their bipotentiality, These findings demonstrate the antigenic profile of clonogenic hepatoblasts and proof of their bipotency, Furthermore, whereas colony formation of adult hepatocytes required epidermal growth factor, clonal growth of hepatoblasts was potentiated without epidermal growth factor. The adult hepatic colonies consisted of RT1A(I+)OX18(+)ICAM-1(++) cells. These results indicate that hepatoblasts possess unique characteristics as compared with adult hepatocytes harboring significant proliferative activity. The phenotypic identity of hepatoblasts and the clonal culture system have relevance for identifying hepatic stem cells from adults, for studying liver development, and for cell therapy based on hepatic progenitors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据