期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 275, 期 43, 页码 33650-33654出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.C000483200
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资金
- NIA NIH HHS [AG14363] Funding Source: Medline
- NIGMS NIH HHS [GM55362] Funding Source: Medline
- NINDS NIH HHS [NS38604] Funding Source: Medline
Prion propagation in transmissible spongiform encephalopathies involves the conversion of cellular prion protein, PrPC, into a pathogenic conformer, PrPSc. Hereditary forms of the disease are Linked to specific mutations in the gene coding for the prion protein. To gain insight into the molecular basis of these disorders, the solution structure of the familial Creutzfeldt-Jakob disease-related E200K variant of human prion protein was determined by multi-dimensional nuclear magnetic resonance spectroscopy, Remarkably, apart from minor differences in flexible regions, the backbone tertiary structure of the E200K variant is nearly identical to that reported for the wild-type human prion protein, The only major consequence of the mutation is the perturbation of surface electrostatic potential. The present structural data strongly suggest that protein surface defects leading to abnormalities in the interaction of prion protein with auxiliary proteins/chaperones or cellular membranes should be considered key determinants of a spontaneous PrPC --> PrPSc conversion in the E200K form of hereditary prion disease.
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