期刊
BIOINFORMATICS
卷 29, 期 5, 页码 656-657出版社
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btt015
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- NHGRI [R01 HG004719]
Motivation: A common question arises at the beginning of every experiment where RNA-Seq is used to detect differential gene expression between two conditions: How many reads should we sequence? Results: Scotty is an interactive web-based application that assists biologists to design an experiment with an appropriate sample size and read depth to satisfy the user-defined experimental objectives. This design can be based on data available from either pilot samples or publicly available datasets.
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