期刊
ANALYTICA CHIMICA ACTA
卷 896, 期 -, 页码 152-159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.09.011
关键词
DNAzyme; Scanometric assay; Colorimetric sensing; Lead ion detection
资金
- Thailand's Office of the Higher Education Commission
- Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission
A facile and simple paper-based scanometric assay was developed to detect Pb2(+) using GR5-DNAzyme. Magnetic beads (MBs) and gold nanoparticles (AuNPs) were used as a signal collector and a signal indicator, respectively. They were linked together by GR5-DNAzyme, comprising an enzyme and a substrate strand pairing up with each other. In the presence of Pb2(+), the substrate strand is cut into two pieces, resulting in the disassembly of AuNPs from the MBs. These AuNPs were spotted on predefined areas on a chromatography paper, where signal is amplified through silver reduction. This sensing platform exhibits high sensitivity and selectivity toward Pb2(+), giving a detection limit of 0.3 nM and a linear fitting range from 0.1 to 1000 nM. Testing of this biosensor in river water and synthetic urine samples also showed satisfying results. Besides offering simultaneous and multi-sample analysis, this paper-based sensing platform presented here could be potentially applied and served as a general platform for on-site, naked eyes, and low-cost monitoring of other heavy metal ions in environmental and body fluid samples. (C) 2015 Elsevier B.V. All rights reserved.
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