期刊
PLANT CELL REPORTS
卷 19, 期 11, 页码 1084-1089出版社
SPRINGER
DOI: 10.1007/s002990000238
关键词
Festuca arundinacea Schreb; Festuca rubra L; transformation; highly regenerative tissue; transgene expression
Highly regenerative tissues of tall fescue and red fescue produced from mature seed-derived embryogenic callus were induced and proliferated on medium containing 2,4-dichlorophenoxyacetic acid (4.5 or 9.0 muM), 6-benzylaminopurine (0, 0.044, 0.44 or 2.2 muM) and cupric sulfate (0.1 or 5.0 muM) under dim-light conditions (10 to 30 muE m(-2) s(-1) 16 h light). Tall fescue tissues were transformed with three plasmids containing the genes for hygromycin phosphotransferase (hpr), phosphinothricin acetyltransferase (bar) and beta -glucuronidase (uidA; gus), and red fescue with three plasmids containing hpt, uidA and a synthetic green fluorescent protein gene [sgfp (S65T)]. DNA from To plants of eight independently transformed lines from tall fescue and 11 from red fescue were analyzed by PCR and DNA blot hybridization. The co-expression frequency of all three transgenes [hpt/bar/uidA or hpt/uidA/sgfp (S65T)] in transgenic tall fescue and red fescue plants was 25-27%; for two transgenes [hpt/bar or hpt/uidA for tall fescue and hpt/uidA or hpt/sgfp(S65T) for red fescue], the co-expression frequency was 50-75%.
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