期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 48, 期 11, 页码 5200-5205出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf000757+
关键词
black tea; polymers; thearubigins; theaflavin; epitheaflavic acid; color; high-speed countercurrent chromatography; HSCCC; TEAC values; Folin-Ciocalteu; polyphenols; flavonoids; antioxidants
Isolation of theaflavins and epitheaflavic acids from black tea using high-speed countercurrent chromatography (HSCCC) on a preparative scale is demonstrated. HSCCC also enabled the isolation of a polymeric fraction from black tea. According to Roberts' classification, the polymeric fraction mainly consisted of SII thearubigins (TR). HPLC analysis showed that the isolated material is free of any known chromatographically resolved tea constituents and eluted from reversed-phase packings as a convex hump (a broad signal). The antioxidant activity of the TR fraction was 3.6 mmol of Trolox equivalents per gram. The total phenolic content of this fraction was determined to be 34.7 g/100 g (as gallic acid equivalents).
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