期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 111, 期 1, 页码 15-30出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-6851(00)00289-9
关键词
apical membrane antigen-1; microneme; Plasmodium; secretion; Toxoplasma
资金
- NCI NIH HHS [CA22435] Funding Source: Medline
- NIAID NIH HHS [AI42355] Funding Source: Medline
A monoclonal antibody (MAb) has been generated against a; novel 63 kDa surface/apical antigen of Toxoplasma gondii tachyzoites which is identified here as TgAMA-1, the Toxoplasma homolog of Plasmodium apical membrane antigen-1 (AMA-1). Sequence analysis, phase partitioning in Triton X-114, and labeling of TgAMA-1 with iodonaphthalene azide all suggest that TgAMA-1 is a type I transmembrane protein. There is a high degree of sequence similarity between TgAMA-1 and Plasmodium AMA-I, most notably in the position of conserved cysteine residues within the protein's predicted extracellular domain. In contrast to full length Plasmodium AMA-1, which has previously been localized to the rhoptries, it is shown here by immunofluorescence and immunoelectron microscopy that intracellular TgAMA-1 is found in the micronemes. A 53 kDa N-terminal proteolytic fragment of TgAMA-1 is constitutively secreted from the parasite at 37 degreesC. As is the case with other microneme proteins, the proteolytic processing and secretion of TgAMA-1 is dramatically enhanced in response to treatments which increase intracellular calcium levels. (C) 2000 Elsevier Science B.V. All rights reserved.
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