期刊
JOURNAL OF ANATOMY
卷 197, 期 -, 页码 529-537出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1046/j.1469-7580.2000.19740529.x
关键词
interleukin-1; tumour necrosis factor-alpha; senescence; ageing; oxidative stress
Sublethal oxidative stresses increase the proportions of human fibroblasts positive for senescence associated beta -galactosidase activity and accelerate the transition in the fibroblast morphotypes characterising fibroblast ageing. Stimulation of fibroblasts with TNF-alpha or IL-1 alpha transiently increases the production of reactive oxygen species (ROS) in human fibroblasts. Here we propose that repeated stimulation of WI-38 fibroblasts with TNF-alpha or IL-1 alpha can generate enough ROS to accelerate the transition in the fibroblast morphotypes and increase the proportion of cells positive for senescence associated beta -galactosidase activity. The involvement of ROS is suggested by experiments where the stimulation of fibroblasts with TNF-alpha or IL-1 alpha are performed in the presence of N-acetylcysteine which increases the intracellular antioxidant potential. It is proposed that the decrease in the proportions of morphotypes I and II, and the increase in the proportions of morphotypes III to VI observed after successive stimulation with TNF-alpha or IL1-alpha is attributed to an increased ROS production occurring during the stimulation.
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