期刊
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
卷 77, 期 11, 页码 1135-1138出版社
AMER OIL CHEMISTS SOC A O C S PRESS
DOI: 10.1007/s11746-000-0178-2
关键词
arachidonic acid; Delta 5 desaturase; DGLA; dihomo-gamma-linolenic acid; Mortierella; sesamin
Dihomo-gamma -linolenic acid (DGLA)-containing oil (triacylglycerol) was produced by the fungus Mortierella alpina S14, which is a Delta5 desaturase-defective mutant of the arachidonic acid-producing strain 1S-4. Using soy flour as the nitrogen source, S14 produced 8.1 g DGLA/L of culture medium in a 50-L jar fermenter. Shifting the cultivation temperature from 26 to 28 degreesC resulted in reduction of the percentage of DGLA in total fatty acids. Under optimal conditions in a 10-kL industrial fermenter, DGLA production reached 7.0 g/L (percentage of DGLA, 43.9%) at day 12 of cultivation. The other fatty acids were palmitic (18.2%), stearic (7.9%), oleic (7.5%), linoleic (4.4%), gamma -linolenic (3.2%), arachidonic (0.4%), and lignoceric (7.7%) acids.
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